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arXiv · 0708.1849

Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy

Ching-Ting Lin·En-Kuang Tien·Szu-Yuan Lee·Long-Sheng Lu·Chau-Chung Wu·Chen-Yuan Dong·Chii-Wann Lin
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Abstract

Ox-LDL uptakes by macrophage play a critical role in the happening of atherosclerosis. Because of its low damage on observed cells and better signal-to- background ratio, two-photon excitation fluorescence microscopy is used to observe NAD(P)H autofluorescence of macrophage under difference cultured conditions- bare cover glass, coated with fibronectin or poly-D-lysine. The results show that the optimal condition is fibronectin coated surface, on which, macrophages profile can be clearly identified on NAD(P)H autofluorescence images collected by two-photon microscopy. Moreover, different morphology and intensities of autofluorescence under different conditions were observed as well. In the future, effects of ox-LDL on macrophages will be investigated by purposed system to research etiology of atherosclerosis.

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Ching-Ting Lin, En-Kuang Tien, Szu-Yuan Lee, Long-Sheng Lu, Chau-Chung Wu, Chen-Yuan Dong, Chii-Wann Lin. 2007-08-14. Effects of Ox-LDL on Macrophages NAD(P)H Autofluorescence Changes by Two-photon Microscopy. https://arxiv.org/abs/0708.1849

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