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arXiv · 0712.1890

Measurement of surface concentration of fluorophores using fluorescence fluctuation spectroscopy

Antoine Delon·Jacques Derouard·Guillaume Delapierre·Rodolphe Jaffiol
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Abstract

Fluorescence fluctuation spectroscopy is applied to study molecules, passing through a small observation volume, usually subjected to diffusive or convective motion in liquid phase. We suggest that such a technique could be used to measure the areal absolute concentration of fluorophores deposited on a substrate or imbedded in a thin film, with a resolution of a few micrometers. The principle is to translate the solid substrate in front of a confocal fluorescence microscope objective and to record the subsequent fluctuations of the fluorescence intensity. The validity of this concept is investigated on model substrates (fluorescent microspheres) and DNA biochips.

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Antoine Delon, Jacques Derouard, Guillaume Delapierre, Rodolphe Jaffiol. 2007-12-12. Measurement of surface concentration of fluorophores using fluorescence fluctuation spectroscopy. https://doi.org/10.1364/ol.31.001142

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