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arXiv · 0903.1121

Quantitative Determination of Spatial Protein-protein Proximity in Fluorescence Confocal Microscopy

Yong Wu·Mansoureh Eghbali·Jimmy Ou·Min Li·Ligia Toro·Enrico Stefani
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Abstract

To quantify spatial protein-protein proximity (colocalization) in fluorescence microscopic images, cross-correlation and autocorrelation functions were decomposed into fast and slowly decaying components. The fast component results from clusters of proteins specifically labeled and the slow one from background/image heterogeneity. We show that the calculation of the protein-protein proximity index and the correlation coefficient are more reliably determined by extracting the fast-decaying component.

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Yong Wu, Mansoureh Eghbali, Jimmy Ou, Min Li, Ligia Toro, Enrico Stefani. 2009-04-28. Quantitative Determination of Spatial Protein-protein Proximity in Fluorescence Confocal Microscopy. https://doi.org/10.1016/j.bpj.2008.12.052

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