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arXiv · 1006.5743

Enzymatic AND Logic Gates Operated Under Conditions Characteristic of Biomedical Applications

Dmitriy Melnikov·Guinevere Strack·Jian Zhou·Joshua Ray Windmiller·Jan Halamek·Vera Bocharova·Min-Chieh Chuang·Padmanabhan Santhosh·Vladimir Privman·Joseph Wang·Evgeny Katz
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Abstract

Experimental and theoretical analyses of the lactate dehydrogenase and glutathione reductase based enzymatic AND logic gates in which the enzymes and their substrates serve as logic inputs are performed. These two systems are examples of the novel, previously unexplored, class of biochemical logic gates that illustrate potential biomedical applications of biochemical logic. They are characterized by input concentrations at logic 0 and 1 states corresponding to normal and abnormal physiological conditions. Our analysis shows that the logic gates under investigation have similar noise characteristics. Both significantly amplify random noise present in inputs, however we establish that for realistic widths of the input noise distributions, it is still possible to differentiate between the logic 0 and 1 states of the output. This indicates that reliable detection of abnormal biomedical conditions is indeed possible with such enzyme-logic systems.

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Dmitriy Melnikov, Guinevere Strack, Jian Zhou, Joshua Ray Windmiller, Jan Halamek, Vera Bocharova, Min-Chieh Chuang, Padmanabhan Santhosh, Vladimir Privman, Joseph Wang, Evgeny Katz. 2010-06-29. Enzymatic AND Logic Gates Operated Under Conditions Characteristic of Biomedical Applications. https://doi.org/10.1021/jp105912e

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