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arXiv · 1102.2632

Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method

Catherine Aude-Garcia·Véronique Collin-Faure·Sylvie Luche·Thierry Rabilloud
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Abstract

Fluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in the literature, staining with ruthenium II tris(bathophenanthroline disulfonate) is particularly cost-effective, but slightly cumbersome owing to difficulties in the preparation of the complex and complexity of staining protocols. We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method.

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Catherine Aude-Garcia, Véronique Collin-Faure, Sylvie Luche, Thierry Rabilloud. 2011-02-13. Improvements and simplifications in in-gel fluorescent detection of proteins using ruthenium II tris-(bathophenanthroline disulfonate): the poor man's fluorescent detection method. https://doi.org/10.1002/pmic.201000370

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