Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

Veikko Linko; Jenni Leppiniemi; Boxuan Shen; Einari Niskanen; Vesa P. Hytönen; J. Jussi Toppari

doi:10.1039/c1nr10518c

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arXiv · 1102.4594

Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules

Veikko Linko·Jenni Leppiniemi·Boxuan Shen·Einari Niskanen·Vesa P. Hytönen·J. Jussi Toppari

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Abstract

We present a method for controlled connection of gold electrodes with dsDNA molecules (locally on a chip) by utilizing polymerase to elongate single-stranded DNA primers attached to the electrodes. Thiol-modified oligonucleotides are directed and immobilized to nanoscale electrodes by means of dielectrophoretic trapping, and extended in a procedure mimicking PCR, finally forming a complete dsDNA molecule bridging the gap between the electrodes. The technique opens up opportunities for building from the bottom-up, for detection and sensing applications, and also for molecular electronics.

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Veikko Linko, Jenni Leppiniemi, Boxuan Shen, Einari Niskanen, Vesa P. Hytönen, J. Jussi Toppari. 2011-02-22. Growth of immobilized DNA by polymerase: bridging nanoelectrodes with individual dsDNA molecules. https://doi.org/10.1039/c1nr10518c

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