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arXiv · 1105.2314

Stimulation of human red blood cells leads to Ca2+-mediated intercellular adhesion

Patrick Steffen·Achim Jung·Duc Bach Nguyen·Torsten Müller·Ingolf Bernhardt·Lars Kaestner·Christian Wagner
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Abstract

Red blood cells (RBCs) are a major component of blood clots, which form physiologically as a response to injury or pathologically in thrombosis. The active participation of RBCs in thrombus solidification has been previously proposed but not yet experimentally proven. Holographic optical tweezers and single-cell force spectroscopy were used to study potential cell-cell adhesion between RBCs. Irreversible intercellular adhesion of RBCs could be induced by stimulation with lysophosphatidic acid (LPA), a compound known to be released by activated platelets. We identified Ca2+ as an essential player in the signaling cascade by directly inducing Ca2+ influx using A23187. Elevation of the internal Ca2+ concentration leads to an intercellular adhesion of RBCs similar to that induced by LPA stimulation. Using single-cell force spectroscopy, the adhesion of the RBCs was identified to be approximately 100 pN, a value large enough to be of significance inside a blood clot or in pathological situations like the vasco-occlusive crisis in sickle cell disease patients.

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Patrick Steffen, Achim Jung, Duc Bach Nguyen, Torsten Müller, Ingolf Bernhardt, Lars Kaestner, Christian Wagner. 2011-05-11. Stimulation of human red blood cells leads to Ca2+-mediated intercellular adhesion. https://arxiv.org/abs/1105.2314

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