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arXiv · 1111.2510

Thermoresponsive micropatterned substrates for single cell studies

Kalpana Mandal·Martial Balland·Lionel Bureau
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Abstract

We describe the design of micropatterned surfaces for single cell studies, based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. A proper choice of the adhesive pattern shapes, combined with the temperature-dependent swelling properties of PNIPAM, allow us to use the polymer brush as a microactuator which induces cell detachment when the temperature is reduced below 32degC.

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Kalpana Mandal, Martial Balland, Lionel Bureau. 2011-11-10. Thermoresponsive micropatterned substrates for single cell studies. https://doi.org/10.1371/journal.pone.0037548

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