Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein

Jean Michel Escoffre; Marie Hubert; Justin Teissié; Marie Pierre Rols; Cyril Favard

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arXiv · 1202.1452

Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein

Jean Michel Escoffre·Marie Hubert·Justin Teissié·Marie Pierre Rols·Cyril Favard

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Abstract

Electropermeabilization is a method by which molecules can be introduced in living cells through the plasma membrane. Here, we show that electropermeabilization affects the lateral mobility of Rae-1, a GPi anchored protein. Our results shows that 10 to 20 % of the membrane surface is occupied by defaults or pores. Gene electrotransfert also affects the lateral mobility of Rae-1. Furthermore, we clearly show that once inserted into the plasma membrane, DNA totaly excludes Rae-1, indicating that DNA molecules are tightly packed together to forms huge aggregates.

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Jean Michel Escoffre, Marie Hubert, Justin Teissié, Marie Pierre Rols, Cyril Favard. 2012-02-07. Effects of electropermeabilization and gene electrotransfert on the lateral mobility of a GPi anchored protein. https://arxiv.org/abs/1202.1452

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