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arXiv · 1202.1975

Observation of Multiphoton-induced Fluorescence from Nano Graphene Oxide and Its Applications in In vitro and In vivo Bioimaging

Jun Qian·Dan Wang·Li Peng·Wang Xi·Fu-Hong Cai·Zhen-Feng Zhu·Hao He·Ming-Lie Hu·Sailing He
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Abstract

In the present paper, we observed both two-photon and three-photon induced distinct photoluminescence from GO nanoparticles under fs laser excitation. Conjugated with PEG molecules, GO nanoparticles exhibited high chemical stability, and could effectively label HeLa cells. Imaged with a two-photon scanning microscope, GO nanoparticles were observed to localize in the mitochondria, endoplasmic reticulum, Golgi and lysosome of HeLa cells. Furthermore, GO nanoparticles were micro-injected into the brain of a black mouse, and in vivo two-photon luminescence imaging illustrated that GO nanoparticles located at 300 {\mu}m depth in the brain could be clearly distinguished.

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Jun Qian, Dan Wang, Li Peng, Wang Xi, Fu-Hong Cai, Zhen-Feng Zhu, Hao He, Ming-Lie Hu, Sailing He. 2012-02-09. Observation of Multiphoton-induced Fluorescence from Nano Graphene Oxide and Its Applications in In vitro and In vivo Bioimaging. https://arxiv.org/abs/1202.1975

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