Effectiveness of a dynein team in tug-of-war helped by reduced load-sensitivity of detachment: evidence from study of bidirectional endosome transport in Dictyostelium discoideum

Abstract

Bidirectional cargo transport by molecular motors in cells is a complex phenomenon, in which the cargo (usually a vesicle) alternately moves in retrograde and anterograde directions. In this case, teams of oppositely pulling motors (eg., kinesin and dynein) bind to the cargo simultaneously, and `coordinate' their activity such that the motion consists of spells of positively and negatively directed segments, separated by pauses of varying duration. A set of recent experiments have analyzed the bidirectional motion of endosomes in the amoeba D. discoideum in detail. It was found that in between directional switches, a team of 5-6 dyneins stall a cargo against a stronger kinesin in tug of war, which lasts for almost a second. As the mean detachment time of a kinesin under its stall load was also observed to be ~ 1s, we infer that the collective detachment time of the dynein assembly must also be similar. Here, we analyze this inference from a modeling perspective, using experimentally measured single-molecule parameters as inputs. We find that the commonly assumed exponential load-dependent detachment rate is inconsistent with observations, as it predicts that a 5-dynein assembly will detach under its combined stall load in less than a hundredth of a second. A modified model where the load-dependent unbinding rate is assumed to saturate at stall-force level for super-stall loads gives results which are in agreement with experimental data. Our analysis suggests that the load-dependent detachment of a dynein in a team is qualitatively different at sub-stall and super-stall loads, a conclusion which is likely to have implications in other situations involving collective effects of many motors.