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arXiv · 1206.3342

Phase gradient microscopy in thick tissue with oblique back-illumination

Tim N. Ford·Kengyeh K. Chu·Jerome Mertz
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Abstract

Phase contrast techniques, such as differential interference contrast (DIC) microscopy, are widely used to provide morphological images of unstained biological samples. The trans-illumination geometry required for these techniques has restricted their application to thin samples. We introduce oblique back-illumination microscopy (OBM), a method of collecting en face phase gradient images of thick scattering samples, enabling near video-rate in vivo phase imaging with a miniaturized probe suitable for endoscopy.

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Tim N. Ford, Kengyeh K. Chu, Jerome Mertz. 2012-06-14. Phase gradient microscopy in thick tissue with oblique back-illumination. https://arxiv.org/abs/1206.3342

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