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arXiv · 1307.2588

Protein Energy Landscapes Determined by 5-Dimensional Crystallography

Marius Schmidt·Vukica Srajer·Robert Henning·Hyotcherl Ihee·Namrta Purwar·Jason Tenboer·Shailesh Tripathi
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Abstract

Free energy landscapes decisively determine the progress of enzymatically catalyzed reactions[1]. Time-resolved macromolecular crystallography unifies transient-state kinetics with structure determination [2-4] because both can be determined from the same set of X-ray data. We demonstrate here how barriers of activation can be determined solely from five-dimensional crystallography [5]. Directly linking molecular structures with barriers of activation between them allows for gaining insight into the structural nature of the barrier. We analyze comprehensive time series of crystal-lographic data at 14 different temperature settings and determine entropy and enthalpy contributions to the barriers of activation. 100 years after the discovery of X-ray scattering, we advance X-ray structure determination to a new frontier, the determination of energy landscapes.

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Marius Schmidt, Vukica Srajer, Robert Henning, Hyotcherl Ihee, Namrta Purwar, Jason Tenboer, Shailesh Tripathi. 2013-07-09. Protein Energy Landscapes Determined by 5-Dimensional Crystallography. https://arxiv.org/abs/1307.2588

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