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arXiv · 1307.3833

Massive Parallelization of STED Nanoscopy Using Optical Lattices

Bin Yang·Frédéric Przybilla·Michael Mestre·Jean-Baptiste Trebbia·Brahim Lounis
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Abstract

Recent developments in stimulated emission depletion (STED) microscopy achieved nanometer scale resolution and showed great potential in live cell imaging. Yet, STED nanoscopy techniques are based on single point-scanning. This constitutes a drawback for wide field imaging, since the gain in spatial resolution requires dense pixelation and hence long recording times. Here we achieve massive parallelization of STED nanoscopy using wide-field excitation together with well-designed optical lattices for depletion and a fast camera for detection. Acquisition of large field of view super-resolved images requires scanning over a single unit cell of the optical lattice which can be as small as 290 nm*290nm. Interference STED (In-STED) images of 2.9 {\mu}m* 2.9 {\mu}m with resolution down to 70 nm are obtained at 12.5 frames per second. The development of this technique opens many prospects for fast wide-field nanoscopy.

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Bin Yang, Frédéric Przybilla, Michael Mestre, Jean-Baptiste Trebbia, Brahim Lounis. 2013-07-15. Massive Parallelization of STED Nanoscopy Using Optical Lattices. https://doi.org/10.1364/oe.22.005581

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