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arXiv · 1308.3172

Realistic simulations reveal extensive sample-specificity of RNA-seq biases

Botond Sipos·Greg Slodkowicz·Tim Massingham·Nick Goldman
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Abstract

In line with the importance of RNA-seq, the bioinformatics community has produced numerous data analysis tools incorporating methods to correct sample-specific biases. However, few advanced simulation tools exist to enable benchmarking of competing correction methods. We introduce the first framework to reproduce the properties of individual RNA-seq runs and, by applying it on several datasets, we demonstrate the importance of accounting for sample-specificity in realistic simulations.

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Botond Sipos, Greg Slodkowicz, Tim Massingham, Nick Goldman. 2013-08-14. Realistic simulations reveal extensive sample-specificity of RNA-seq biases. https://arxiv.org/abs/1308.3172

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