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arXiv · 1312.3673

Lag, lock, sync, slip: the many "phases" of coupled flagella

Kirsty Y. Wan·Kyriacos C. Leptos·Raymond E. Goldstein
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Abstract

In a multitude of life's processes, cilia and flagella are found indispensable. Recently, the biflagellated chlorophyte alga Chlamydomonas has become a model organism for the study of ciliary coordination and synchronization. Here, we use high-speed imaging of single pipette-held cells to quantify the rich dynamics exhibited by their flagella. Underlying this variability in behaviour, are biological dissimilarities between the two flagella - termed cis and trans, with respect to a unique eyespot. With emphasis on the wildtype, we use digital tracking with sub-beat-cycle resolution to obtain limit cycles and phases for self-sustained flagellar oscillations. Characterizing the phase-synchrony of a coupled pair, we find that during the canonical swimming breaststroke the cis flagellum is consistently phase-lagged relative to, whilst remaining robustly phase-locked with, the trans flagellum. Transient loss of synchrony, or phase-slippage, may be triggered stochastically, in which the trans flagellum transitions to a second mode of beating with attenuated beat-envelope and increased frequency. Further, exploiting this alga's ability for flagellar regeneration, we mechanically induced removal of one or the other flagellum of the same cell to reveal a striking disparity between the beating of the cis vs trans flagellum, in isolation. This raises further questions regarding the synchronization mechanism of Chlamydomonas.

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Kirsty Y. Wan, Kyriacos C. Leptos, Raymond E. Goldstein. 2013-12-12. Lag, lock, sync, slip: the many "phases" of coupled flagella. https://doi.org/10.1098/rsif.2013.1160

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