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arXiv · 1402.0172

Modeling and Optimization of Genetic Screens via RNA Interference and FACS

Yair Goldberg·Yuval Nov
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Abstract

We study mathematically a method for discovering which gene is related to a cell phenotype of interest. The method is based on RNA interference -- a molecular process for gene deactivation -- and on coupling the phenotype with fluorescence in a FACS machine. A small number of candidate genes are thus isolated, and then tested individually. We model probabilistically this process, prove a limit theorem for its outcome, and derive operational guidelines for maximizing the probability of successful gene discovery.

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Yair Goldberg, Yuval Nov. 2014-02-02. Modeling and Optimization of Genetic Screens via RNA Interference and FACS. https://doi.org/10.1017/s0269964814000254

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