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arXiv · 1403.2833

Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope

Charles-Edouard Leroux·Alexei Grichine·Irène Wang·Antoine Delon
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Abstract

We describe the effect of optical aberrations on fluorescence fluctuations microscopy (FFM), when focusing through a single living cell. FFM measurements are performed in an aqueous fluorescent solution and prove to be a highly sensitive tool to assess the optical aberrations introduced by the cell. We demonstrate an adaptive optics (AO) system to remove the aberration-related bias in the FFM measurements. Our data show that AO is not only useful when imaging deep in tissues but also when performing FFM measurements through a single cellular layer. This work paves the way for the application of FFM to complex three-dimensional multicellular samples.

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Charles-Edouard Leroux, Alexei Grichine, Irène Wang, Antoine Delon. 2014-03-12. Correction of cell-induced optical aberrations in a fluorescence fluctuation microscope. https://arxiv.org/abs/1403.2833

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