Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics

Adam H Marblestone; Evan R Daugharthy; Reza Kalhor; Ian D Peikon; Justus M Kebschull; Seth L Shipman; Yuriy Mishchenko; Je Hyuk Lee; Konrad P Kording; Edward S Boyden; Anthony M Zador; George M Church

arXiv · 1404.5103

Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics

Adam H Marblestone·Evan R Daugharthy·Reza Kalhor·Ian D Peikon·Justus M Kebschull·Seth L Shipman·Yuriy Mishchenko·Je Hyuk Lee·Konrad P Kording·Edward S Boyden·Anthony M Zador·George M Church

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Abstract

We propose a neural connectomics strategy called Fluorescent In-Situ Sequencing of Barcoded Individual Neuronal Connections (FISSEQ-BOINC), leveraging fluorescent in situ nucleic acid sequencing in fixed tissue (FISSEQ). FISSEQ-BOINC exhibits different properties from BOINC, which relies on bulk nucleic acid sequencing. FISSEQ-BOINC could become a scalable approach for mapping whole-mammalian-brain connectomes with rich molecular annotations.

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Adam H Marblestone, Evan R Daugharthy, Reza Kalhor, Ian D Peikon, Justus M Kebschull, Seth L Shipman, Yuriy Mishchenko, Je Hyuk Lee, Konrad P Kording, Edward S Boyden, Anthony M Zador, George M Church. 2014-04-21. Rosetta Brains: A Strategy for Molecularly-Annotated Connectomics. https://arxiv.org/abs/1404.5103

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