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arXiv · 1407.3668

Expression proteomics reveals protein targets and highlights mechanisms of action of small molecule drugs

Alexey Chernobrovkin·Consuelo Marin Vicente·Neus Visa·Roman A. Zubarev
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Abstract

Phenomenological screening of small molecule libraries for anticancer activity yields potentially interesting candidate molecules, with a bottleneck in the determination of drug targets and the mechanism of anticancer action. A novel approach to drug target deconvolution compares the abundance profiles of proteins expressed in a panel of cells treated with different drugs, and identifies proteins with cell-type independent and drug-specific regulation that is exceptionally strong in relation to the other proteins. Mapping top candidates on known protein networks reveals the mechanism of drug action, while abundant proteins provide a signature of cellular death/survival pathways. The above approach can significantly shorten drug target identification, and thus facilitate the emergence of novel anticancer treatments.

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Alexey Chernobrovkin, Consuelo Marin Vicente, Neus Visa, Roman A. Zubarev. 2014-07-14. Expression proteomics reveals protein targets and highlights mechanisms of action of small molecule drugs. https://doi.org/10.1038/srep11176

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