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arXiv · 1412.3712

In situ fiber-optical monitoring of cytosolic calcium in tissue explant cultures

Manuel Ryser·Lisa Künzi·Marianne Geiser·Martin Frenz·Jaro Rička
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Abstract

We present a fluorescence-lifetime based method for monitoring cell and tissue activity in situ, during cell culturing and in the presence of a strong autofluorescence background. The miniature fiber-optic probes are easily incorporated in the tight space of a cell culture chamber or in an endoscope. As a first application we monitored the cytosolic calcium levels in porcine tracheal explant cultures using the Calcium Green-5N (CG5N) indicator. Despite the simplicity of the optical setup we are able to detect changes of calcium concentration as small as 2.5 nM, with a monitoring time resolution of less than 1 s.

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Manuel Ryser, Lisa Künzi, Marianne Geiser, Martin Frenz, Jaro Rička. 2014-12-11. In situ fiber-optical monitoring of cytosolic calcium in tissue explant cultures. https://doi.org/10.1002/jbio.201300089

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