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arXiv · 1506.06039

moco: Fast Motion Correction for Calcium Imaging

Alexander Dubbs·James Guevara·Darcy S. Peterka·Rafael Yuste
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Abstract

Motion correction is the first in a pipeline of algorithms to analyze calcium imaging videos and extract biologically relevant information, for example the network structure of the neurons therein. Fast motion correction would be especially critical for closed-loop activity triggered stimulation experiments, where accurate detection and targeting of specific cells in necessary. Our algorithm uses a Fourier-transform approach, and its efficiency derives from a combination of judicious downsampling and the accelerated computation of many $L_2$ norms using dynamic programming and two-dimensional, fft-accelerated convolutions. Its accuracy is comparable to that of established community-used algorithms, and it is more stable to large translational motions. It is programmed in Java and is compatible with ImageJ.

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Alexander Dubbs, James Guevara, Darcy S. Peterka, Rafael Yuste. 2015-06-19. moco: Fast Motion Correction for Calcium Imaging. https://arxiv.org/abs/1506.06039

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