Topological defect launches 3D mound in the active nematic sheet of neural progenitors

Abstract

Cultured stem cells have become a standard platform not only for regenerative medicine and developmental biology but also for biophysical studies. Yet, the characterization of cultured stem cells at the level of morphology and macroscopic patterns resulting from cell-to-cell interactions remain largely qualitative, even though they are the simplest features observed in everyday experiments. Here we report that neural progenitor cells (NPCs), which are multipotent stem cells that give rise to cells in the central nervous system, rapidly glide and stochastically reverse its velocity while locally aligning with neighboring cells, thus showing features of an active nematic system. Within the two-dimensional nematic pattern, we find interspaced topological defects with +1/2 and -1/2 charges. Remarkably, we identified rapid cell accumulation leading to three-dimensional mounds at the +1/2 topological defects. Single-cell level imaging around the defects allowed quantification of the evolving cell density, clarifying that not only cells concentrate at +1/2 defects, but also escape from -1/2 defects. We propose the mechanism of instability around the defects as the interplay between the anisotropic friction and the active force field, thus addressing a novel universal mechanism for local cell density control.