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arXiv · 1605.06737

An unbiased metric of antiproliferative drug effect in vitro

Leonard A. Harris·Peter L. Frick·Shawn P. Garbett·Keisha N. Hardeman·B. Bishal Paudel·Carlos F. Lopez·Vito Quaranta·Darren R. Tyson
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Abstract

In vitro cell proliferation assays are widely used in pharmacology, molecular biology, and drug discovery. Using theoretical modeling and experimentation, we show that current antiproliferative drug effect metrics suffer from time-dependent bias, leading to inaccurate assessments of parameters such as drug potency and efficacy. We propose the drug-induced proliferation (DIP) rate, the slope of the line on a plot of cell population doublings versus time, as an alternative, time-independent metric.

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Leonard A. Harris, Peter L. Frick, Shawn P. Garbett, Keisha N. Hardeman, B. Bishal Paudel, Carlos F. Lopez, Vito Quaranta, Darren R. Tyson. 2016-05-22. An unbiased metric of antiproliferative drug effect in vitro. https://doi.org/10.1038/nmeth.3852

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