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arXiv · 1610.05451

Questioning the reliability of estimates of enzyme inhibitor constant: Case of competitive inhibition

Sharmistha Dhatt
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Abstract

Reliability of kinetic parameters are crucial in understanding enzyme kinetics within cellular system. The present study suggests a few cautions that need introspection for estimation of parameters like K(M), V(max) and K(I) using Lineweaver-Burk plots. The quality of IC(50) too needs a thorough reinvestigation because of its direct link with K(I) and K(M) values. Inhibition kinetics under both steady-state and non-steady-state conditions are studied and errors in estimated parameters are compared against actual values to settle the question of their adequacy.

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Sharmistha Dhatt. 2016-10-18. Questioning the reliability of estimates of enzyme inhibitor constant: Case of competitive inhibition. https://arxiv.org/abs/1610.05451

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