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arXiv · 1611.00287

Structured illumination microscopy with unknown patterns and a statistical prior

Li-Hao Yeh·Lei Tian·Laura Waller
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Abstract

Structured illumination microscopy (SIM) improves resolution by down-modulating high-frequency information of an object to fit within the passband of the optical system. Generally, the reconstruction process requires prior knowledge of the illumination patterns, which implies a well-calibrated and aberration-free system. Here, we propose a new \textit{algorithmic self-calibration} strategy for SIM that does not need to know the exact patterns {\it a priori}, but only their covariance. The algorithm, termed PE-SIMS, includes a Pattern-Estimation (PE) step requiring the uniformity of the sum of the illumination patterns and a SIM reconstruction procedure using a Statistical prior (SIMS). Additionally, we perform a pixel reassignment process (SIMS-PR) to enhance the reconstruction quality. We achieve 2$\times$ better resolution than a conventional widefield microscope, while remaining insensitive to aberration-induced pattern distortion and robust against parameter tuning.

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Li-Hao Yeh, Lei Tian, Laura Waller. 2017-01-12. Structured illumination microscopy with unknown patterns and a statistical prior. https://doi.org/10.1364/boe.8.000695

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