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arXiv · 1702.03582

Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy

Shwetadwip Chowdhury·Will J. Eldridge·Adam Wax·Joseph A. Izatt
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Abstract

Sub-diffraction resolution imaging has played a pivotal role in biological research by visualizing key, but previously unresolvable, sub-cellular structures. Unfortunately, applications of far-field sub-diffraction resolution are currently divided between fluorescent and coherent-diffraction regimes, and a multimodal sub-diffraction technique that bridges this gap has not yet been demonstrated. Here we report that structured illumination (SI) allows multimodal sub-diffraction imaging of both coherent quantitative-phase (QP) and fluorescence. Due to the conventional fluorescent applications of SI, we first demonstrated the principle of SI-enabled three-dimensional (3D) QP sub-diffraction imaging with calibration microspheres. Image analysis confirmed enhanced lateral and axial resolutions over diffraction-limited QP imaging, and established striking parallels between coherent SI and conventional optical diffraction tomography. We next introduce an optical system utilizing SI to achieve 3D sub-diffraction, multimodal QP/fluorescent visualization of A549 biological cells fluorescently tagged for F-actin. Our results suggest that SI has unique utility in studying biological phenomena with significant molecular, biophysical, and biochemical components.

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Shwetadwip Chowdhury, Will J. Eldridge, Adam Wax, Joseph A. Izatt. 2017-02-12. Structured illumination multimodal 3D-resolved quantitative phase and fluorescence sub-diffraction microscopy. https://doi.org/10.1364/boe.8.002496

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