Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals

Salah Awel; Richard A. Kirian; Max O. Wiedorn; Kenneth R. Beyerlein; Nils Roth; Daniel A. Horke; Dominik Oberthür; Juraj Knoska; Valerio Mariani; Andrew Morgan; Luigi Adriano; Alexandra Tolstikova; P. Lourdu Xavier; Oleksandr Yefanov; Andrew Aquila; Anton Barty; Shatabdi Roy-Chowdhury; Mark S. Hunter; Daniel James; Joseph S. Robinson; Uwe Weierstall; Andrei V. Rode; Saša Bajt; Jochen Küpper; Henry N. Chapman

doi:10.1107/s1600576717018131

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arXiv · 1702.04014

Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals

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Abstract

We demonstrate near-atomic-resolution Bragg diffraction from aerosolized single granulovirus crystals using an x-ray free-electron laser. The form of the aerosol injector is nearly identical to conventional liquid-microjet nozzles, but the x-ray-scattering background is reduced by several orders of magnitude by the use of helium carrier gas rather than liquid. This approach provides a route to study the weak diffuse or lattice-transform signal arising from small crystals. The high speed of the particles is particularly well suited to upcoming MHz-repetition-rate x-ray free-electron lasers.

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Salah Awel, Richard A. Kirian, Max O. Wiedorn, Kenneth R. Beyerlein, Nils Roth, Daniel A. Horke, Dominik Oberthür, Juraj Knoska, Valerio Mariani, Andrew Morgan, Luigi Adriano, Alexandra Tolstikova, P. Lourdu Xavier, Oleksandr Yefanov, Andrew Aquila, Anton Barty, Shatabdi Roy-Chowdhury, Mark S. Hunter, Daniel James, Joseph S. Robinson, Uwe Weierstall, Andrei V. Rode, Saša Bajt, Jochen Küpper, Henry N. Chapman. 2017-02-13. Femtosecond x-ray diffraction from an aerosolized beam of protein nanocrystals. https://doi.org/10.1107/s1600576717018131

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