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arXiv · 1702.05163

Conjugate adaptive optics with remote focusing for three-dimensional focusing through scattering media

Xiaodong Tao·Tuwin Lam·Bingzhao Zhu·Qinggele Li·Marc R. Reinig·Joel Kubby
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Abstract

The small correction volume for conventional wavefront shaping methods limits their applications in biological imaging through scattering media. We demonstrate large volume wavefront shaping through a scattering layer with a single correction by conjugate adaptive optics and remote focusing (CAORF). The remote focusing module can keep the conjugation between the AO and scattering layer during three-dimensional scanning. This new configuration provides a wider correction volume by the best utilization of the memory effect in a fast three-dimensional laser scanning microscope. Our results show that the proposed system can provide 10 times wider axial field of view compared with a conventional conjugate AO system when 16,384 segments are used on a spatial light modulator. We also demonstrated three-dimensional fluorescence imaging and multi-spot patterning through a scattering layer.

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Xiaodong Tao, Tuwin Lam, Bingzhao Zhu, Qinggele Li, Marc R. Reinig, Joel Kubby. 2017-02-16. Conjugate adaptive optics with remote focusing for three-dimensional focusing through scattering media. https://arxiv.org/abs/1702.05163

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