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arXiv · 1706.02261

Biobeam - Rigorous wave-optical simulations of light-sheet microscopy

Martin Weigert·Eugene W. Myers·Moritz Kreysing
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Abstract

Sample-induced image-degradation remains an intricate wave-optical problem in light-sheet microscopy. Here we present biobeam, an open-source software package that enables to simulate operational light-sheet microscopes by combining data from 10^5 - 10^6 multiplexed and GPU-accelerated point-spread-function calculations. The wave-optical nature of these simulations leads to the faithful reproduction of spatially varying aberrations, diffraction artifacts, geometric image distortions, adaptive optics, and emergent wave optical phenomena, and renders image-formation in light-sheet microscopy computationally tractable.

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BibTeXRIS

Martin Weigert, Eugene W. Myers, Moritz Kreysing. 2017-06-06. Biobeam - Rigorous wave-optical simulations of light-sheet microscopy. https://doi.org/10.1371/journal.pcbi.1006079

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