A New Framework for Determination of Excitatory and Inhibitory Conductances Using Somatic Clamp

Abstract

The interaction between excitation and inhibition is crucial for brain computation. To understand synaptic mechanisms underlying brain function, it is important to separate excitatory and inhibitory inputs to a target neuron. In the traditional method, after applying somatic current or voltage clamp, the excitatory and inhibitory conductances are determined from the synaptic current-voltage (I-V) relation --- the slope corresponds to the total conductance and the intercept corresponds to the reversal current. Because of the space clamp effect, the measured conductance in general deviates substantially from the local conductance on the dendrite. Therefore, the interpretation of the conductance measured by the traditional method remains to be clarified. In this work, based on the investigation of an idealized ball-and-stick neuron model and a biologically realistic pyramidal neuron model, we first demonstrate both analytically and numerically that the conductance determined by the traditional method has no clear biological interpretation due to the neglect of a nonlinear interaction between the clamp current and the synaptic current across the spatial dendrites. As a consequence, the traditional method can induce an arbitrarily large error of conductance measurement, sometimes even leads to unphysically negative conductance. To circumvent the difficulty of elucidating synaptic impact on neuronal computation using the traditional method, we then propose a framework to determine the effective conductance that reflects directly the functional impact of synaptic inputs on action potential initiation and thereby neuronal information processing. Our framework has been further verified in realistic neuron simulations, thus greatly improves upon the traditional approach by providing a reliable and accurate assessment of the role of synaptic activity in neuronal computation.