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arXiv · 1710.10340

Photobleaching of randomly-rotating fluorescently-decorated particles

Swadhin Taneja·Andrew D. Rutenberg
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Abstract

Randomly rotating particles that have been isotropically labeled with rigidly linked fluorophores will undergo non-isotropic (patchy) photobleaching under illumination due to the dipole coupling of fluorophores with light. For a rotational diffusion rate $D$ of the particle and a photobleaching timescale $\tau$ of the fluorophores, the dynamics of this process are characterized by the dimensionless combination $D \tau$. We find significant interparticle fluctuations at intermediate $D \tau$. These fluctuations vanish at both large and small $D \tau$, or at small or large elapsed times $t$. Associated with these fluctuations between particles, we also observe transient non-monotonicities of the brightness of individual particles. These non-monotonicities can be as much 20\% of the original brightness. We show that these novel photobleach-fluctuations dominate over variability of single-fluorophore orientation when there are at least $10^3$ fluorophores on individual particles.

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Swadhin Taneja, Andrew D. Rutenberg. 2017-10-27. Photobleaching of randomly-rotating fluorescently-decorated particles. https://doi.org/10.1063/1.4989673

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