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arXiv · 1802.10080

Simulation of live-cell imaging system reveals hidden uncertainties in cooperative binding measurements

Masaki Watabe·Satya N. V. Arjunan·Wei Xiang Chew·Kazunari Kaizu·Koichi Takahashi
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Abstract

We propose a computational method to quantitatively evaluate the systematic uncertainties that arise from undetectable sources in biological measurements using live-cell imaging techniques. We then demonstrate this method in measuring biological cooperativity of molecular binding networks: in particular, ligand molecules binding to cell surface receptor proteins. Our results show how the non-statistical uncertainties lead to invalid identification of the measured cooperativity. Through this computational scheme, the biological interpretation can be more objectively evaluated and understood under a specific experimental configuration of interest.

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Masaki Watabe, Satya N. V. Arjunan, Wei Xiang Chew, Kazunari Kaizu, Koichi Takahashi. 2018-02-27. Simulation of live-cell imaging system reveals hidden uncertainties in cooperative binding measurements. https://doi.org/10.1103/physreve.100.010402

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