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arXiv · 1806.01067

Generalized quantification of three-dimensional resolution in optical diffraction tomography using the projection of maximal spatial bandwidths

Chansuk Park·Seungwoo Shin·Yongkeun Park
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Abstract

Optical diffraction tomography (ODT) is a three-dimensional (3D) quantitative phase imaging technique, which enables the reconstruction of the 3D refractive index (RI) distribution of a transparent sample. Due to its fast, non-invasive, and quantitative 3D imaging capability, ODT has emerged as one of the most powerful tools for various applications. However, the spatial resolution of ODT has only been quantified along the lateral and axial directions for limited conditions; it has not been investigated for arbitrary-oblique directions. In this paper, we systematically quantify the 3D spatial resolution of ODT by exploiting the spatial bandwidth of the reconstructed scattering potential. The 3D spatial resolution is calculated for various types of ODT systems, including the illumination-scanning, sample-rotation, and hybrid scanning-rotation methods. In particular, using the calculated 3D spatial resolution, we provide the spatial resolution as well as the arbitrary sliced angle. Furthermore, to validate the present method, the point spread function of an ODT system is experimentally obtained using the deconvolution of a 3D RI distribution of a microsphere and is compared with the calculated resolution. The present method for defining spatial resolution in ODT is directly applicable for various 3D microscopic techniques, providing a solid criterion for the accessible finest 3D structures.

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Chansuk Park, Seungwoo Shin, Yongkeun Park. 2018-06-04. Generalized quantification of three-dimensional resolution in optical diffraction tomography using the projection of maximal spatial bandwidths. https://doi.org/10.1364/josaa.35.001891

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