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arXiv · 1812.02334

Detection of Proteoglycan Loss from Articular Cartilage using Brillouin Microscopy, with Applications to Osteoarthritis

Pei-Jung Wu·Maryam Imani Masouleh·Daniele Dini·Carl Paterson·Peter Torok·Darryl R. Overby·Irina V. Kabakova
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Abstract

The degeneration of articular cartilage (AC) occurs in osteoarthritis (OA), which is a leading cause of pain and disability in middle-aged and older people. The early disease related changes in cartilage extra-cellular matrix (ECM) start with depletion of proteoglycan(PG), leading to an increase in tissue hydration and permeability. These early compositional changes are small (<10%) and hence difficult to register with conventional non-invasive imaging technologies (magnetic resonance and ultrasound imaging). Here we apply Brillouin microscopy for detecting changes in the mechanical properties and composition of porcine AC. OA-like degradation is mimicked by enzymatic tissue digestion, and we compare Brillouin microscopy measurements against histological staining of PG depletion over varying digestion times and enzyme concentrations. The non-destructive nature of Brillouin imaging technology opens new avenues for creating minimally invasive arthroscopic devices for OA diagnostics and therapeutic monitoring.

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Pei-Jung Wu, Maryam Imani Masouleh, Daniele Dini, Carl Paterson, Peter Torok, Darryl R. Overby, Irina V. Kabakova. 2018-12-06. Detection of Proteoglycan Loss from Articular Cartilage using Brillouin Microscopy, with Applications to Osteoarthritis. https://arxiv.org/abs/1812.02334

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