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arXiv · 1909.02148

Automated and optimally FRET-assisted structural modeling

Mykola Dimura·Thomas O. Peulen·Hugo Sanabria·Dmitro Rodnin·Katherina Hemmen·Claus A. M. Seidel·Holger Gohlke
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Abstract

FRET experiments can yield state-specific structural information on complex dynamic biomolecular assemblies. However, FRET experiments need to be combined with computer simulations to overcome their sparsity. We introduce (i) an automated FRET experiment design tool determining optimal FRET pairs for structural modeling, (ii) a protocol for efficient FRET-assisted computational structural modeling at multiple scales, and (iii) a quantitative quality estimate for judging the accuracy of determined structures. We tested against simulated and experimental data.

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Mykola Dimura, Thomas O. Peulen, Hugo Sanabria, Dmitro Rodnin, Katherina Hemmen, Claus A. M. Seidel, Holger Gohlke. 2019-09-04. Automated and optimally FRET-assisted structural modeling. https://doi.org/10.1016/j.bpj.2018.11.1815

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