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arXiv · 1909.12893

Continuous focal translation enhances rate of point-scan volumetric microscopy

Courtney Johnson·Jack Exell·Jonathon Kuo·Kevin Welsher
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Abstract

Two-Photon Laser-Scanning Microscopy is a powerful tool for exploring biological structure and function because of its ability to optically section through a sample with a tight focus. While it is possible to obtain 3D image stacks by moving a stage, this perframe imaging process is time consuming. Here, we present a method for an easy to implement and inexpensive modification of an existing two-photon microscope to rapidly image in 3D using an electrically tunable lens to create a tessellating scan pattern which repeats with the volume rate. Using appropriate interpolating algorithms, the volumetric imaging rate can be increased by a factor up to four-fold. This capability provides the expansion of the two-photon microscope into the third dimension for faster volumetric imaging capable of visualizing dynamics on timescales not achievable by traditional stage stack methods.

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Courtney Johnson, Jack Exell, Jonathon Kuo, Kevin Welsher. 2019-09-09. Continuous focal translation enhances rate of point-scan volumetric microscopy. https://doi.org/10.1364/oe.27.036241

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