arXiv · 2005.09881
Towards nanoelectromechanical photothermal localization microscopy
Abstract
Single-molecule microscopy has become an indispensable tool for biochemical analysis. The capability of characterizing distinct properties of individual molecules without averaging has provided us with a different perspective for the existing scientific issues and phenomena. Recently, the super-resolution fluorescence microscopy techniques have overcome the optical diffraction limit by the localization of molecule positions. However, the labelling process can potentially modify the intermolecular dynamics. Based on the highly-sensitive nanomechanical photothermal microscopy reported previously, we propose optimizations on this label-free microscopy technique towards localization microscopy. A localization precision of 3 $\unicode{x212B}$ is achieved with gold nanoparticles, and the detection of polarization-dependent absorption is demonstrated, which opens the door for further improvement with polarization modulation imaging.
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Miao-Hsuan Chien, Silvan Schmid. 2020-05-20. Towards nanoelectromechanical photothermal localization microscopy. https://arxiv.org/abs/2005.09881
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