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arXiv · 2009.03278

Cryopreservation of pineapple (Ananas comosus) apices

M. T. Gonzalez-Arnao·M. M. Ravelo·C. U. Villavicencio·M. E. Martinez-Montero·F. Engelmann
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Abstract

The encapsulation-dehydration and vitrification techniques were experimented for freezing apices of pineapple in vitro plantlets. Positive results were achieved using vitrification only. Optimal conditions included a 2-d preculture of apices on medium supplemented with 0.3M sucrose, loading treatment for 25 min in medium with 0.75M sucrose + 1M glycerol and dehydration with PVS2 vitrification solution at 0 degrees C for 7 h before rapid freezing in liquid nitrogen. This method resulted in 65, 35 and 25% survival with apices of varieties Puerto Rico, Perolera and Smooth Cayenne, respectively. Recovery of cryopreserved apices took place directly, without transitory callus formation.

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M. T. Gonzalez-Arnao, M. M. Ravelo, C. U. Villavicencio, M. E. Martinez-Montero, F. Engelmann. 2020-08-29. Cryopreservation of pineapple (Ananas comosus) apices. https://arxiv.org/abs/2009.03278

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