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arXiv · 2009.07606

Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy

George Dwapanyin·Dirk Spangenberg·Alexander Heidt·Thomas Feurer·Gurthwin Bosman·Pieter Neethling·Erich Rohwer
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Abstract

Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, I$^2$PIE, a novel spectral phase control technique, was implemented in order to compress broad-bandwidth supercontinuum light pulses generated in an all-normal-dispersion (ANDi) photonic crystal fiber (PCF). The technique, based on time-domain ptychography, is demonstrated here in a nonlinear microscopy application for the first time, to the best of our knowledge. The first real-world application of this technique for second-harmonic generation and two-photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, I$^2$PIE leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy.

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George Dwapanyin, Dirk Spangenberg, Alexander Heidt, Thomas Feurer, Gurthwin Bosman, Pieter Neethling, Erich Rohwer. 2020-09-16. Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy. https://doi.org/10.1364/josab.396938

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