Parameter estimation in FACS-seq enables high-throughput characterization of phenotypic heterogeneity

Huibao Feng; Chong Zhang

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arXiv · 2010.03317

Parameter estimation in FACS-seq enables high-throughput characterization of phenotypic heterogeneity

Huibao Feng·Chong Zhang

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Abstract

Phenotypic heterogeneity is a most fascinating property of a population of cells, which shows the differences among individuals even with the same genetic background and extracellular environmental conditions. However, the lack of high-throughput analysis of phenotypic diversity has limited our research progress. To deal with it, we constructed a novel parameter estimation method in FACS-seq, a commonly used experimental framework, to achieve simultaneous characterization of thousands of variants in a library. We further demonstrated the model's ability in estimating the expression properties of each variant, which we believe can help to decipher the mechanisms of phenotypic heterogeneity.

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Huibao Feng, Chong Zhang. 2020-10-07. Parameter estimation in FACS-seq enables high-throughput characterization of phenotypic heterogeneity. https://arxiv.org/abs/2010.03317

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