A fit-less approach to the elasticity of the handles in optical tweezers experiments

Alessandro Mossa; Ciro Cecconi

doi:10.1007/s00249-022-01603-2

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arXiv · 2111.10119

A fit-less approach to the elasticity of the handles in optical tweezers experiments

Alessandro Mossa·Ciro Cecconi

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Abstract

The elastic properties of the double-stranded DNA handles used in optical tweezers experiments on biomolecules are customarily modeled by an extensible worm-like chain model. Fitting such model to experimental data however is no trivial task, as the function depends on four parameters in a highly non-linear fashion. We hereby propose a method to bypass the fitting procedure and obtain an empirical force vs. extension curve that accurately reproduce the elasticity of the handles.

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Alessandro Mossa, Ciro Cecconi. 2021-11-19. A fit-less approach to the elasticity of the handles in optical tweezers experiments. https://doi.org/10.1007/s00249-022-01603-2

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