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arXiv · 2208.09451

Guided-deconvolution for Correlative Light and Electron Microscopy

Fengjiao Ma·Rainer Kaufmann·Jaroslaw Sedzicki·Zoltán Cseresnyés·Christoph Dehio·Stephanie Hoeppener·Marc Thilo Figge·Rainer Heintzmann
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Abstract

Correlative light and electron microscopy is a powerful tool to study the internal structure of cells. It combines the mutual benefit of correlating light (LM) and electron (EM) microscopy information. However, the classical approach of overlaying LM onto EM images to assign functional to structural information is hampered by the large discrepancy in structural detail visible in the LM images. This paper aims at investigating an optimized approach which we call EM-guided deconvolution. It attempts to automatically assign fluorescence-labelled structures to details visible in the EM image to bridge the gaps in both resolution and specificity between the two imaging modes.

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Fengjiao Ma, Rainer Kaufmann, Jaroslaw Sedzicki, Zoltán Cseresnyés, Christoph Dehio, Stephanie Hoeppener, Marc Thilo Figge, Rainer Heintzmann. 2022-08-19. Guided-deconvolution for Correlative Light and Electron Microscopy. https://arxiv.org/abs/2208.09451

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