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arXiv · 2212.04176

DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent biological samples

Isabell Whiteley·Chenchen Song·Glenn A Howe·Thomas Knöpfel·Christopher J Rowlands
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Abstract

A targeted imaging system has been developed for applications requiring recording from stationary samples at high spatiotemporal resolutions. It works by illuminating regions of interest in rapid sequence, and recording the signal from the whole field of view onto a single photodetector, and can be implemented at low cost on an existing microscope without compromising existing functionality. The system is characterized in terms of speed, spatial resolution, and tissue penetration depth, before being used to record individual action potentials from ASAP-3 expressing neurons in an ex vivo mouse brain slice preparation.

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Isabell Whiteley, Chenchen Song, Glenn A Howe, Thomas Knöpfel, Christopher J Rowlands. 2022-12-08. DIRECT, a low-cost system for high-speed, low-noise imaging of fluorescent biological samples. https://arxiv.org/abs/2212.04176

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