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arXiv · 2303.14233

A computer vision based optical method for measuring fluid level in cell culture plates

Pierre V. Baudin·Mircea Teodorescu
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Abstract

For a transparent well with a known volume capacity, changes in fluid level result in predictable changes in magnification of an overhead light source. For a given well size and fluid, the relationship between volume and magnification can be calculated if the fluid's index of refraction is known or in a naive fashion with a calibration procedure. Light source magnification can be measured through a camera and processed using computer vision contour analysis with OpenCV. This principle was applied in the design of a 3D printable sensing device using a raspberry pi zero and a camera

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Pierre V. Baudin, Mircea Teodorescu. 2023-03-24. A computer vision based optical method for measuring fluid level in cell culture plates. https://doi.org/10.1371/journal.pone.0290951

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