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arXiv · 2304.01456

Fluorescence Microscopy: a statistics-optics perspective

Mohamadreza Fazel·Kristin S. Grussmayer·Boris Ferdman·Aleksandra Radenovic·Yoav Shechtman·Jörg Enderlein·Steve Pressé
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Abstract

Fundamental properties of light unavoidably impose features on images collected using fluorescence microscopes. Modeling these features is ever more important in quantitatively interpreting microscopy images collected at scales on par or smaller than light's wavelength. Here we review the optics responsible for generating fluorescent images, fluorophore properties, microscopy modalities leveraging properties of both light and fluorophores, in addition to the necessarily probabilistic modeling tools imposed by the stochastic nature of light and measurement.

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Mohamadreza Fazel, Kristin S. Grussmayer, Boris Ferdman, Aleksandra Radenovic, Yoav Shechtman, Jörg Enderlein, Steve Pressé. 2023-04-04. Fluorescence Microscopy: a statistics-optics perspective. https://arxiv.org/abs/2304.01456

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