Microfluidics Generation of Millimeter-sized Matrigel Droplets

Abstract

Significant progress has been made to increase access to droplet microfluidics for labs with limited microfluidics expertise or fabrication equipment. In particular, using off-the-shelf systems has been a valuable approach. However, the ability to modify a channel design and, thus, the functional characteristics of the system is of great value. In this work, we describe the development of co-flow microfluidics and their fabrication methods for generating uniform millimeter-sized (0.5 - 2 mm) hydrogel droplets. Two complementary approaches based on desktop CO2 laser cutting were developed to prototype and build durable co-flow droplet microfluidics. After demonstrating the co-flow systems, water-in-oil experiments and dimensionless number analysis were used to examine the operational characteristics of the system. Specifically, the Capillary number analysis indicated that millimeter-sized droplet generators operated in the desirable geometry-controlled regime despite their length scales being larger than traditional microfluidics systems. Next, the tunable generation of Matrigel droplets was demonstrated. By adjusting the relative flow rates, the droplet size could be tuned. Finally, we demonstrated fibroblast encapsulation and cell viability for up to 7 days as a proof-of-concept experiment. The systems presented are simple and effective tools to generate robust hydrogel droplets and increase the accessibility of this technology to teaching labs or research settings with limited resources or access to microfluidics.