Bottom-Up Approach to Explore Alpha-Amylase Assisted Membrane Remodelling

Harshit Kumar; Sayar Mandal; Suhasi Gupta; Hemraj Meena; Mayur Kadu; Rajni Kudawla; Pratibha Sharma; Indu Pal Kaur; John H Ipsen; Tripta Bhatia

arXiv · 2308.00339

Bottom-Up Approach to Explore Alpha-Amylase Assisted Membrane Remodelling

Harshit Kumar·Sayar Mandal·Suhasi Gupta·Hemraj Meena·Mayur Kadu·Rajni Kudawla·Pratibha Sharma·Indu Pal Kaur·John H Ipsen·Tripta Bhatia

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Abstract

Soluble alpha-amylases play an important role in the catabolism of polysaccharides. In this work, we show that the enzyme can interact with the lipid membrane and further alter its mechanical properties. Vesicle fluctuation spectroscopy is used for quantitative measurement of the membrane bending rigidity of phosphatidylcholines lipid vesicles from the shape fluctuation based on the whole contour of Giant Unilamellar Vesicles (GUVs). The bending rigidity of the lipid vesicles of 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine in water increases significantly with the presence of 0.14 micromolar alpha-amylase in the exterior solution. However, as the concentration increases above 1 micromolar, the bending rigidity decreases but remains higher than estimated without the protein. Contact between the alpha-amylase in the outer solution and the outer leaflet leads to spontaneous membrane curvature and the corresponding morphological changes of the GUVs. The presence of outbuds directly demonstrates that AA has a preferable interaction with the membrane, giving a positive spontaneous curvature of $C_0 \leq 0.05 \ \mu \rm{m}^{-1}$ at $18 \ \mu$M $(\approx$ 1 mg/ml) of AA concentration. Above 1 mg/ml of AA concentration the shape of GUVs collapse completely suggesting a highly convoluted state.

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Harshit Kumar, Sayar Mandal, Suhasi Gupta, Hemraj Meena, Mayur Kadu, Rajni Kudawla, Pratibha Sharma, Indu Pal Kaur, John H Ipsen, Tripta Bhatia. 2023-08-01. Bottom-Up Approach to Explore Alpha-Amylase Assisted Membrane Remodelling. https://arxiv.org/abs/2308.00339

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