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arXiv · 2310.15728

On the minimal model of kinetic cooperativity. The case of glucokinase

Leonid Christophorov
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Abstract

The minimal 3-state scheme of kinetic cooperativity of monomeric enzymes is subjected to detailed analysis. The rigorous criteria of positive cooperativity and its sigmoidal version are established in terms of the system parameters (rate constants). It is shown that the cooperativity extent is especially sensitive to the rates and direction of the exchange between conformational states of the free enzyme. However, no necessity of the "kinetic resonance" (or, moreover, its generality claimed recently) for enhancing cooperativity is revealed. Overall, while the minimal 3-state model serves well for qualitative understanding the origin of kinetic cooperativity, it is hardly suitable for quantitative describing reactions of real enzymes, as it is shown with the case of glucokinase.

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Leonid Christophorov. 2023-10-24. On the minimal model of kinetic cooperativity. The case of glucokinase. https://arxiv.org/abs/2310.15728

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