Phases, morphologies, and transitions in a membrane model for the endoplasmic reticulum

Abstract

We introduce a novel model, comprising self-avoiding surfaces and incorporating edges and tubules, that is designed to characterize the structural morphologies and transitions observed within the endoplasmic reticulum (ER). By employing discretized models, we model smooth membranes with triangulated surfaces, and we utilize numerical variational methods to minimize energies associated with periodic morphologies. Our study obtains phases, their morphologies, and their transitions and examines their dependence on the membrane chemical potential, the line tensions, and the positive Gaussian curvature stiffness. By starting with diverse topological structures, we explore shape variations by using Surface Evolver, while maintaining fixed topology. Notably, we identify the region of parameter space where the model displays lamellae, with a lattice of helical edges connecting the layers; this resembles structures that have been observed in the rough ER. Furthermore, our investigation reveals an intricate phase diagram with periodic structures, including flat lamellar sheets, sponge phases, and configurations comprising tubules with junctions, which are akin to the morphology of the smooth ER. An estimation of lattice parameters is achieved through fluctuation analysis. Significantly, our model predicts a transition between homotopically equivalent lamellae, with helical edges and configurations featuring tubules with junctions.